Natural and synthetic rubbers are widely used for various applications because of their unusual properties such as high elasticity, extensibility, resiliency. Natural rubber and synthetic rubbers have an unsaturation in their repeating unit of polymer chain. Due to this they get degraded by influence of heat, light, oxygen, ozone, fatigue, metal ions poisons and flexing. Antioxidants are mainly amine derivatives such as 1,2-dihydro-2,2,4-trimethylquinoline (TMQ) and they function by reacting with oxides or broken polymer chain ends caused by reaction with oxygen. They therefore prevent the propagation of oxidative degradation, enabling the retention of the physical properties of the elastomer. Used polymers are generally disposed to the environments or in some case are being recycled. Therefore, optimization of additives is required for the performance of the characteristic properties of the rubber and to control the release of various additives to the environment. The present work is focused on studying the effect of concentration of 1,2-dihydro-2,2,4-trimethylquinoline (TMQ) with alkyl derivative of p-Phenylene diamine on the physical and mechanical properties of natural rubber like tensile strength, fatigue to failure and ozone resistant capacity. Concentration of TMQ was varied from 0 to 2.5 phr to study the optimization rubber characteristic.